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神經(jīng)營養(yǎng)因子3(NT3)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)

ELISA Kit DIY Materials for Neurotrophin 3 (NT3)

NTF3; HDNF; NGF2; Nerve Growth Factor 2; Neurotrophic Factor

  • 神經(jīng)營養(yǎng)因子3(NT3)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)產品包裝(模擬)
  • 神經(jīng)營養(yǎng)因子3(NT3)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)產品包裝(模擬)
  • Certificate通過ISO 9001、ISO 13485質量體系認證

特異性

試劑盒中的抗體對神經(jīng)營養(yǎng)因子3(NT3)具有高靈敏度和高特異性,經(jīng)檢測與其它相似物質無明顯交叉反應。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標板即可用于ELISA實驗,后續(xù)使用方法與ELISA同。

儲存

抗體,標準品和HRP標記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質期為1年,試劑一旦開封,在4度保存時間為1個月。

配套試劑

參考文獻

雜志參考文獻
SCaracteriza[Teses: Disponiveis]
Canadian Journal of Physiology and PharmacologyProtective effects of telmisartan and tempol on lipopolysaccharide-induced cognitive impairment, neuroinflammation, and amyloidogenesis: possible role of brain-derived neurotrophic factor[pubmed:28388365]
Efecto de Diferentes Intervenciones de Ejercicio Físico Agudo Sobre Niveles Plasmáticos de BDNF, NT-3 y NT-4 en Adultos con Sobrepeso[:]
Artificial Cells Nanomedicine and BiotechnologyHistological and functional outcomes in a rat model of hemisected spinal cord with sustained VEGF/NT-3 release from tissue-engineered grafts[Pubmed: 31899965]
laboratory investigationMacrophage-derived neurotrophin-3 promotes heterotopic ossification in rats[Pubmed: 31896816]
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BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRYSea urchin gangliosides exhibit neuritogenic effects in neuronal PC12 cells via TrkA- and TrkB-related pathways[33589896]
Clin EpigeneticsGenome?wide profiling of DNA methylation and gene expression unravel the epigenetic landscape in diabetes-related hypothyroidism[34092241]
J Nutr Sci Vitaminol (Tokyo)Effects of a Tea Cultivar “MK5601” on Behaviors and Hippocampal Neurotrophin-3 Levels in Middle-Aged Mice[34193676]
Iranian Journal of Basic Medical SciencesAdrenomedullin increases cAMP accumulation and BDNF expression in rat DRG and spinal motor neurons[34712429]
Molecular Biology ReportsUmbilical cord-derived mesenchymal stem cell conditioned medium reverses neuronal oxidative injury by inhibition of TRPM2 activation and the JNK signaling?…[Pubmed:35585377]